Generate Panel

Generate Building Blocks

On the Generate Building Blocks page, the user can import or upload amino acid (aa) Fv sequences and assemble selected Building Blocks through an interactive UI. Sequences can be brought in from multiple sources, including uploading from an external workflow via “Upload Sequences,” importing from Luma by clicking “Import Luma Sequences,” or importing from BioGlyph by selecting “Import BioGlyph Sequences.”

Using “Upload Sequences,” the user can currently select from different sequence types, including Fvs, VHHs, and Muteins. Additionally, the user must select the target from a drop-down menu automatically generated during project creation, as well as specify the species.

After uploading a .FASTA file, a table displaying the Fv sequences is generated. Next, the user clicks “Upload Sequences” at the bottom to proceed.

Like the “Variant Analyzer,” after clicking “Upload Sequences,” the sequences undergo a validation process, just like any other sequences entering the BioGlyph database for the first time.

The user will have the opportunity to review and accept or reject any sequences that have been flagged.

Once all sequences are imported or uploaded into the project, the user can begin generating Building Blocks. In the example below, the user selects the Fv sequences along with the CH1 and CL components needed to create the Fab Building Block.

Generation of the scFv Building Block by selecting CD19 Fv sequences and the linker component.

Generation of Fc Building Block by selecting each component: “Hinge,” “CH2,” and “CH3.”

Examples of Building Block subpanels generated—one subpanel per Building Block. Click “Submit Building Blocks” to proceed.

Next, the user can review all Building Blocks generated and/or imported into this request before clicking “Save Building Blocks to Request.

Below is an example of importing Building Blocks (e.g., muteins) for this request from the BioGlyph database.

See below the status of your request on the “Request Control” page. For example, the user can confirm that all five Building Blocks required to generate the designed formats are now available.

Generate MsAb Panel

Clicking on the task will take the user to the “Generate MsAb Panel” page

Here, the user has full control over selecting each Building Block within the same type to associate with each format. In the example below, for the mAb format (BG0001), all 69 PD1 Fabs and one homo Fc were selected. The subpanel on the right-hand side shows the selections.

Example using the trispecific BG0007 format.

Selected Building Blocks can be assigned to each format based on specific molecular attributes.

Selection of remaining Building Blocks. Linkers connecting Building Blocks can be imported from the components library or designed directly within the request.

Once all selected formats have been associated with Building Blocks and Linkers, click “Generate Panel.”

Next, using the plate view options, users can review all molecules generated by format, as well as the total unique sequences that make up each sequence set encoding the molecules in the panel.

Click on plate wells to view each molecule details, including the format, Building Blocks, sequence set, and sequence annotations.

Users can also switch to the table view to quickly see the sequence sets alongside biophysical data calculated for each molecule, including MW and pI.

Example data file format containing amino acid sequences