Variant Analyzer

Currently this task can only handle Fv sequences. The user will upload a .FASTA file containing the selected Fv sequences and specify the target specificity. Please note that the target association is entirely user-defined and is not verified by BioGlyph.

Upload File

table with the Fv sequences uploaded into the Variant Analyzer

Upload Sequences

When you upload or import any protein sequence(s) into the BioGlyph database, a validation process is performed to ensure the integrity of the sequences.

In the example above, all uploaded Fv sequences were successfully validated. Below is an example of sequences that failed validation, along with the subsequent steps to address the issues.

Four Fv sequences failed the BioGlyph validation process. At this stage, the user can review each “Failed” Fv and, in some cases, override the initial classification—for example, by accepting the five-residue N-term deletion in the VH sequence shown below.

If, after reviewing all flags within a sequence or sequence set (such as an Fv), the user chooses to accept them, the Fv’s status will be updated to “Completed” and it will be ready for import into the BioGlyph database. See the updated status below.

Importing

At this stage, the sequences are stored in the BioGlyph database and ready for use by the scientist. Below are the four analytical tools available in the “Variant Analyzer.”

Analysis Result Submission Table with residue level annotations

Multiple Sequence Alignment

Phylogenetic tree

Dimensionality Reduction

On the Request Control Page, you can view the Fv sequences imported into REQ001.

If you have multiple files, GitBook makes it easy to import full repositories too — allowing you to keep your GitBook content in sync.