Protein Characterization

The production of biologics is often supported by in silico characterization tools to evaluate both the likelihood of generating the intended molecule and the formation of byproducts. Additionally, after production, mass spectrometry is used to assess the integrity of the intended molecule. To support these efforts, we have developed the following tools: Sequence Mapping & Detect Mass

Sequence Mapping

Scientists can utilize high-resolution 2D format representations to map amino acids at various levels, from full-length amino acid chains down to single residues. This capability supports a wide range of applications, including mapping single-point mutations, ADC conjugation sites, non-canonical amino acids, and peptides. It is particularly useful for protein engineers and molecular assessment scientists.

First Page – select Format. Molecules are organized under protein formats.

Next Page – select Molecule

Sequence mapping page with 2D high resolution. On top right buttons for save molecule and Save session as are available.

In the same page below, you see the associated sequence set with three levels of annotation: building block, component and regions.

Sequence analyzes is represented in the 2D format and at sequence level.

The 3D viewer can be activated to display the 3D structure for each building block in the molecule

Detect Mass

This tool allows scientists to input intact or reduced (still in development) mass determinations, after which the software generates the two chain combinations (candidate #1 and #2) closest to the input mass observed MW (Obs MW) with one or more formats per candidate, providing valuable insights into molecular structure. First Page – select Format. Molecules are organized under protein formats

Next Page – select Molecule

Type in the observed MW from the LCMS analyzes.

Results page – two candidates each with a sequence set and corresponding format are offered. Candidate #1 will be automatically registered to the database.